Con A Agarose

• Cat.No.: Con A-001A
• Product Overview: Con A agarose is an affinity medium with concanavalin A (Con A) coupled to agarose 4B by the cyanogen bromide method, which is a?chromatography?medium?for?separation?and?purification?of?glycoproteins,?polysaccharides,?and?glycolipids.

Specification

• Tag: Non
• Cat.no: Con A-001A
• Characteristics: ConA binds specifically α-D-mannosyl and α-D-glucosyl residues in terminal position of ramified structures from B-Glycans. It has 4 binding sites, corresponding to the 4 sub-units. The molecular weight is 104-112KDa and the isoelectric point (pI) is in the range of 4.5-5.5. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to agarose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.
• Size: 5 mL, 100 mL
• Matrix: 4% agarose
• Functional group: Con A
• Average particle size: 90μm
• Ligand: Con A
• Ligand concentration: 10?to?15?mg?Con?A/ml?medium
• Dynamic binding capacity: 20-45?mg?porcine?thyroglobulin/ml?medium
• Recommended flow rate: 75 cm/h
• Recommended column height: 5‐20cm
• Chemical stability: Stable?in?all?commonly?used?aqueous?buffers.?Chelating?agents?such?as?EDTA,?8?M?urea,?or?solutions?having?pH?below?3?should?be?avoided?as?these?conditions?result?in?removal?of?manganese?from?the?lectin?with?loss?of?activity as a result.
• pH working range: 4-9
• Warning: Avoid using Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions results in removal of manganese from the lectin with loss of activity as a result.