Cinnamon Alcohol Dehydrogenase Activity assay kit

• Cat.No.: KITZ-087
• Product Overview: Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) is a key enzyme in plant secondary metabolism, particularly in lignin synthesis, and is closely related to plant growth, development, and defense against pathogen invasion. This kit provides a simple, sensitive, and rapid method for measuring the activity of CAD. CAD catalyzes the conversion of cinnamyl alcohol and NADP+ to cinnamaldehyde and NADPH. The product reacts with a specific chromogenic agent to produce a colored substance. By measuring the rate of increase in the colored substance, the activity of the CAD enzyme can be calculated.

Specification

• Size: 96 Samples
• Storage: 4°C
• Shipping: Ice pack
• Kit Components: Extraction Solution: Liquid, 120mL × 1 bottle, stored at 4°C.
  Reagent 1: Liquid, 5mL × 1 bottle, stored at 4°C.
  Reagent 2: Liquid × 1 vial, stored at 4°C.
  Reagent 3: Powder, mg × 1 bottle, stored at 4°C.
  Before use, add 14mL of Reagent 4 to fully dissolve; any unused reagent should be stored at 4°C.
  Reagent 4: Liquid, 15mL × 1 bottle, stored at 4°C.
  Standard: Powder, mg × 1 vial, stored at 4°C.
  This reagent is used if a new calibration curve needs to be prepared.
• Materials Required but Not Supplied: Microplate reader, 96-well plate, benchtop centrifuge, adjustable pipette, mortar, ice, and distilled water.