Chromatin Immunoprecipitation (ChIP) Kit (For Animal)

• Cat.No.: KITMI-CP03A
• Product Overview: Chromatin Immunoprecipitation (ChIP) is used to investigate whether proteins and DNA are interacted in the body. This technique helps researchers determine which histone repairs occur at a particular location in the genome in the nucleus adorn. Using specific antibody and antigen interaction, the DNA fragment bound to the target protein can be deposited, which can truly reflect the binding in DNA-binding proteins on DNA sequences.

Specification

• Size: 6 tests
• Storage: Box A components: Store at 4°C
  Box B components: Store at -20°C
• Applications: Study transcription factors or histone modifications
• Notes: 6 tests mean 6 times of single group (1 group of IP or 1 group of IgG or 1 group of positive antibody) immunoprecipitation experiments. The following procedure includes 1 group of IgG, 1 group of IP and/or 1 group of positve control (Options), which consumes 2-3 test reagents
• Shipping: On Ice
• Kit Components: Box A components
  5×PBS: 7mL;
  Glycine buffer: 700uL
  Lysis Buffer: 1.4mL
  ChIP Buffer: 14mL
  DTT: 35uL
  ProteinA/G Magnetic Beads: 350uL
  Elution buffer: 1mL
  5M NaCl: 150uL
  
  Box B components
  Wash Buffer 1: 7mL
  Wash Buffer 2: 7mL
  Wash Buffer 3: 7mL
  Wash Buffer 4: 14mL
  Proteinase K: 48uL
  Normal Rabbit IgG: 6uL
  Normal Mouse IgG: 6uL
  Positive antibody (Histone-H3): 6uL
  5 uM GAPDH primer (human): 40uL
  5 uM GAPDH primer (mouse): 40uL
  Protease Inhibitor (100×): 70uL
  RNase A: 24uL
  
  
  
  
  
• Materials Required but Not Supplied: 37% formaldehyde or 16% formaldehyde
  Qubit fluorometer and quantitative kit
  DNA purification recovery kit
  SYBR Green qPCR Mix
  DNA library building kit