CHK2 Kinase Assay Kit

• Cat.No.: KITZ-042
• Product Overview: CHK2 (checkpoint kinase 2, also known as CHEK2) is an evolutionarily conserved serine/threonine protein kinase that operates as a cell cycle checkpoint in response to DNA damage. Thus, it is rapidly phosphorylated and activated upon DNA damage. It is known to phosphorylate and inactivate phosphatase Cdc25C, thereby preventing entry into mitosis. CHK2 also stabilizes tumor suppressor TP53 and is a key player in the ATM DNA damage response and repair pathway. CHK2 mutations are observed in Li-Fraumeni syndrome, an inherited predisposition to cancer. Mutations in CHK2 are also linked to various spontaneous types of cancer. The CHK2 Kinase Assay Kit is designed to measure CHK2 kinase activity for screening and profiling applications using ADP-Glo as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CHK2 kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Specification

• Size: 96 reactions
• Storage: Store at -80 °C
• Shipping: Dry ice
• Kit Components: CHK2: 2.5 μg -80°C
  5x Kinase assay buffer: 1.5 ml -20°C
  ATP (500 μM): 100 μl -20°C
  CHKtide (1 mg/ml): 500 μl -20°C
  96-well plate, white 1 Room Temperature
• Materials Required but Not Supplied: ADP-Glo Kinase Assay
  Microplate reader capable of reading luminescence
  Adjustable micropipettor and sterile tips
  30°C incubator