CHK1 Kinase Assay Kit

• Cat.No.: KITZ-041
• Product Overview: CHK1 is an evolutionarily conserved serine/threonine protein kinase that operates as a cell cycle checkpoint at the G2/M phase transition in response to DNA damage. Activated CHK1 triggers a pleiotropic cellular response via phosphorylation of downstream effectors. For example, Chk1 activates and phosphorylates Wee1 kinase and regulates Cdc25C in response to DNA damage in G2 phase. Its activity is highly controlled at the G2/M phase transition. The CHK1 Kinase Assay Kit is designed to measure CHK1 kinase activity for screening and profiling applications using ADP-Glo as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CHK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Specification

• Size: 96 reactions
• Storage: Store at -80 °C
• Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS)
  applications.
• Shipping: Dry ice
• Kit Components: CHK1: 5 μg -80°C
  5x Kinase assay buffer: 1.5 ml -20°C
  ATP (500 μM): 100 μl -20°C
  CHKtide (1 mg/ml): 500 μl -20°C
  96-well plate, white 1 Room Temp.
• Materials Required but Not Supplied: ADP-Glo Kinase Assay
  Microplate reader capable of reading luminescence
  Adjustable micropipettor and sterile tips
  30°C incubator