Cathepsin G Activity Colorimetric Assay Kit

• Cat.No.: Kit-0183
• Product Overview: Simple one-step procedure; takes only 1 hour
  Fast, robust and convenient
  Cathepsin-G assay is straightforward, and can be adapted to 96-well plate assays.

Specification

• Description: Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In Cathepasin G Activity assay Kit, Cathepsin G will cleave the substrate and release the dye group, pNA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable for the activity of Cathepsin G.
• Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin G proteases that can initiate or propagate proapoptotic signals.
• Usage: For Research Use Only! Not For Use in Humans
• Storage: -20°C
• Size: 100 assays
• Kit Components: • Assay Buffer
  • Substrate
  • Cathepsin G Inhibitor
  • pNA Standard (0.1 M)
  • Positive Control
• Target Species: Mammalian
• Detection method: Absorbance (405 nm)
• Compatible Sample Types: Cell and tissue lysates, serum
• Tag: Non