Caspase-4 Colorimetric Assay Kit

• Cat.No.: Kit-1118

Specification

• Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
• Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
• Storage: -20°C
• Shipping: Gel Pack
• Kit Components: Cell Lysis Buffer; 2X Reaction Buffer; LEVD-pNA (4 mM); DTT (1 M); Dilution Buffer
• Target Species: Mammalian
• Detection method: Absorbance (400or 405 nm)
• Features & Benefits: Simple one-step procedure; takes only 1-2 hours;
  Fast and convenient;
  Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent Caspase activity.
• Tag: Non