Caspase-3 Immunoassay Kit (Fluorometric)
Cat.No. : | Kit-2305 |
Product Overview : | Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT; Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected. |
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Tag : | Non |
Applications : | Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. |
Storage : | -20°C |
Shipping : | Gel Pack |
Size : | 100 assays |
Kit Components : | Cell Lysis Buffer; Coating Buffer; Anti-Caspase-3 Antibody (20X); Blocking Buffer; Incubation Buffer; DTT (1 M); DEVD-AFC Substrate (1 mM); Positive Control (rh-Caspase-3); Microtiter Plate; Adhesive Plate Cover |
Target Species : | Mammalian |
Detection method : | Fluorescence(Ex/Em 370-425/ 490-525nm) |
Features & Benefits : | Simple one-step procedure; takes only ~3 hours; Fast and convenient |
Not For Human Consumption!
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