Boronate PMMA Affinity Resin

• Cat.No.: Boronate-1P
• Product Overview: Boronate PMMA Affinity Resin is based on a homogeneous hydrophilic monodisperse polymethacrylate. 3-aminophenylboric acid is surface bonded with fillers for the purification or analysis of compounds with cis-diol groups, such as glycoproteins, nucleic acids and sugars. The particle size of the filler is 60 μm, good pressure resistance, good physical and chemical stability, and the surface of the filler is highly hydrophilic, avoiding non-specific adsorption with biological samples. The dextran exclusion limit of fillers is about 2x106, and the exclusion limit of spherical proteins is about 5x106, which can be used for industrial purification.

Specification

• Bead diameter: 60 μm
• Matrix: Homogeneous hydrophilic polymethacrylate
• Binding capacity: 10-30 μmol sorbitol/mL filler
• Maximum linear velocity: 1800 cm/h
• Operating temperature: ≤40°C
• pH: 2-12
• Operating pressure: ≤1 Mpa(10 bar)
• Storage Buffer: 50% (v/v), stored in 20% ethanol or 2% benzyl alcohol, 2-8°C
• Mobile phase compatibility: 1. Capacity with aqueous solution, water and acetonitrile, acetone or methanol mixture. Typical buffer solution: phosphate, acetate and HEPES, MES, buffer salt system without primary amine.
  2. The use of buffers with pH < 6.5 or reagents containing cis-diol may affect adsorption.
• Storage: 2-8°C
• Reconstitution: 2.0-3.0 M NaCI