Boronate Affinity Resin, Highly-crosslinked Agarose

• Cat.No.: Boronate-2A
• Product Overview: Boronate Affinity Resin, Highly-crosslinked Agarose is an affinity chromatography medium formed by coupling 3-aminobenzenoboric acid with specific spacer arms designed on the surface of a new generation of highly rigid agarose gel microsphere skeleton. Phenylborate ligands can form borates with 1, 2-cis-diol through reversible covalent bonding under weakly basic conditions, and can specifically bind substances containing 1, 2-cis-diol structure, including glycoproteins (including antibodies), enzymes, polysaccharides, nucleosides, nucleotides and catechols. NADP+ or ATP can also be used to assist the separation and purification of target molecules that cannot be directly bound to the filler.

Specification

• Bead diameter: 30~100 μm
• Matrix: High rigidity agarose gel
• Ligand: 3-aminophenylboric acid, ~20 μmol/mL
• Binding capacity: ~10 mg IgG/mL
• Maximum linear velocity: >1500 cm/h, 0.5 MPa
• Average grain diameter: ~60 μm
• Recommended operating velocity: 150 cm/h
• pH: 3~10 (operation process), 2~13 (CIP process)
• Storage: 20% ethanol, 2~8°C (storage), 2~30°C(transportation)