Beta-Lactamase Inhibitor Screening Kit

• Cat.No.: KITZ-062
• Product Overview: β-Lactamase (beta-lactamase, βL, EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. β-Lactamases specifically hydrolyze β-lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.
  The β-Lactamase Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of β-Lactamase inhibitors. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present.

Specification

• Size: The kit is sufficient for 100 assays in 96 well plates.
• Storage: The kit is shipped on wet ice. Storage at -20°C, protected from light, is recommended.
• Shipping: Ice pack
• Kit Components: β-Lactamase Assay Buffer 25 mL
  Nitrocefin, in DMSO 0.1 mL
  β-Lactamase 1 vl
  Inhibitor Control, Clavulanic acid 1 vl
• Materials Required but Not Supplied: 96 well flat-bottom plate. It is recommended to use clear plates for colorimetric assays.
  Spectrophotometric multiwell plate reader
  Dimethyl sulfoxide (DMSO)