Asparaginase Free Cell Medium for HEK Cell Lines

• Cat.No.: Media-01
• Product Overview: Asparaginase-Free Medium is a chemically defined, animal-component-free medium suitable for high-density suspension culture of HEK293 cells following transient transfection. The main components include glutamine, HEPES, sodium bicarbonate, amino acids, vitamins, and trace elements.
  Asparaginase-Free Medium is specifically applied to:
  • Suspension culture of HEK293 cells
  • Transient transfection of HEK293 cells for protein/antibody expression, lentivirus, etc.
  • High-density culture of HEK293 cells and scale-up in bioreactors
  

Specification

• Storage: 2~8°C, protected from light
• Endotoxin: Low
• Notes: For scientific research and cell culture production use only
• Product Features: Product Features
  • Supports suspension culture and transient transfection of HEK293 cells
  • Ready-to-use, no additional supplements required
  • Animal-component-free, chemically defined
  • Consistently recommended by cell culture experts
  
• Cell Line: 293
• Glutamine: Yes
• Phenol Red: Phenol Red
• Buffer System: Sodium Bicarbonate
• Concentration: 1×; 4×
• Culture Type: Suspension
• Product Type: Chemically Defined, Protein-Free, Animal-Component-Free
• Shelf Life: 12 months
• Shipping: Ice Pack
• Usage Protocol: • Allow the medium to warm to room temperature before use.
  • For transfection, passage 293 cells in 1× CBM293 medium, with a recommended minimum of 3 passages for adaptation, in suspension culture in shaking flasks or bioreactors, with a seed cell passage density greater than 0.3×10^6 cells/mL. (Shaking bed speed reference: 150-180 rpm)
  • Grow 293 seed cells to a density of 1.5-3.0×10^6 cells/mL, dilute the cell density to 0.8-1.0×10^6 cells/mL with 1× fresh CBM293 medium, and continue to culture the cells for 24 hours or less until the density reaches 1.5-3.0×10^6 cells/mL (not exceeding 3.0×10^6 cells/mL), then proceed with transfection.
  • After 24 hours post-transfection, add supplement CBM-feed to the culture medium at a volume ratio of 2% (e.g., 2mL to 100mL), add once daily. Alternatively, add at 4% every two days.
  • Typically, harvest and purify after 7 days post-transfection or when cell viability drops below 55%.
  
• Fig: Media-01.png