Antibody/Protein Aggregation Assay Kit

• Cat.No.: Kit-1088

Specification

• Tag: Non
• Description: Antibody and protein aggregation are important issues in the field of Biotherapeutics. A wide range of conditions and different stages of processing of biopharmaceuticals, such as, formulation, purification, storage, shipping and handling, heat, freeze thaw cycles, mechanical stress, chemical trea;ent etc. might cause aggregation of proteins, enzymes and antibody targets. The possibility of protein aggregates in eliciting immunogenicity is a major concern for biotherapeutics, which might compromise the safety and efficacy of the biologically derived drug products. Recombinant protein production and storage would highly benefit from a high throughput screening method that can screen a variety of formulation and storage conditions of a particular target proteintibody. Currently, different biophysical techniques such as Dynamic Light Scattering (DLS), Size-exclusion Chromatography with Multi-angle Light Scattering (SEC-MALS) can detect the presence of small amount of aggregates in a proteintibody preparation, but they are not amenable for high-throughput screening format. Antibody/Protein Aggregation assay kit is a platform to estimate the extent of aggregation within a proteintibody sample. This is a fast, high-throughput, plate-based fluorometric assay format and can detect as low as 0.5% antibody aggregate.
• Applications: Measurement of protein aggregation for protein formulation
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: Aggregation Assay Buffer; Antibody aggregate standard (0%); Antibody aggregate standard (10%); Aggregation Assay Probe
• Detection method: Detection method- Fluorescence (Ex/Em = 440/500 nm)
• Features & Benefits: Simple one-step procedure; takes less than one hour;
  High-throughput compatible;
  The entire assay can be performed directly in a 96-well plate and does not require washing/long incubation times or solubilization steps