Alcohol Dehydrogenase Detection Kit

• Cat.No.: Kit-0052
• Product Overview: Alcohol Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.

Specification

• Tag: Non
• Description: Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
• Applications: Functional Studies
• Storage: Store the kit at -20°C, protect from light. Allow ADH Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.DEVELOPER: Reconstitute Developer with 0.9 ml of ddH2O. Pipette up and down several times to completely dissolve the pellet into solution. (Do not vortex).
• Kit Components: Components Identifier 100 testsADH Assay Buffer WM 1 x 25mlADH Positive Control (Lyophilized) Green 1 vialDeveloper (Lyophilized) Red 1 vialIsopropanol Blue 1 x 1mlNADH Standard (Lyophilized) Yellow 1 vial
• Compatible Sample Types: Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media
• Sensitivity: 0.01 mU/well