ADP/ATP Ratio Bioluminescence Assay Kit

• Cat.No.: Kit-2306
• Product Overview: ADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

Specification

• Tag: Non
• Applications: Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.
• Storage: The kit is shipped on ice. Store all reagents at -20°C.
• Size: 100 tests
• Kit Components: Assay Buffer: 10 mL Substrate: 120 µL Cosubstrate 120 µL ATP Enzyme: 120 µL ADP Enzyme: 120 µL
• Target Species: Mammalian
• Detection method: Luminometer or Beta Counter.
• Features & Benefits: Simple one-step procedure; takes only 30 minutes;
  Fast and convenient;
  The ADP/ATP ratio assay offers highly consistent results and with excellent correlation to other apoptosis markers (e.g. TUNEL-based assays and caspase assays). In addition the assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 cells/well).
• Materials Required but Not Supplied: Pipetting devices, centrifuge tubes, white flat-bottom 96-well plates, luminescence plate reader.