Active Spike Trimer protein-coupled magnetic Beads

• Cat.No.: Spike-4123M

Specification

• Species: COVID-19
• Appearance: Powder mixture
• Particle size: 2 μm
• Source: HEK293
• Protein length: Val 16 - Pro 1213
• Bio-activity: Immobilized 35 μg Spike Trimer protein to 1 mg Beads, can bind the Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 with an EC50 of 0.5499 μg/mL.
• Coupled amount of protein: ≈251 pmol (35 μg) Spike Trimer/mg Beads
• Capacity: >133 pmol (20 μg) antibody/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike trimer protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. The SARS-CoV-2 Spike trimer protein, His Tag, Super stable trimer (SPN-C52H9) is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This very first SARS-CoV-2 Spike trimer protein-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.