Active Spike Trimer (P.1) protein-coupled magnetic Beads

• Cat.No.: Spike-4129M

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Protein Length: Val 16 - Pro 1213(L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F, R683A, R685A,F817P, A892P, A899P, A942P, K986P, V987P)
• Appearance: Powder mixture
• Particle size: 2 μm
• Bio-activity: Immobilized 40μg S protein trimer/1mg beads can bind the Anti-S1 Antibody with an EC50 of 0.5720μg/mL.
• Coupled amount of protein: ≈286 pmol (40 μg) SARS-CoV-2 Spike Trimer (P.1)/mg beads
• Capacity: >242 pmol (36 μg) Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The SARS-CoV-2 Spike Trimer (P.1) Coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike trimer to streptavidin-conjugated magnetic beads. The spike proteins coupled to the beads contain 12 mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) identified in the Brazilian variant (known as P.1). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.