Active Spike Trimer(Delta) protein-coupled magnetic Beads

• Cat.No.: Spike-4134M

Specification

• Species: COVID-19
• Appearance: Powder mixture
• Particle size: 2 μm
• Source: HEK293
• Protein length: Arg 319 - Lys 537(T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)
• Bio-activity: Immobilized 46.8 μg SARS-CoV-2 (Delta) Spike Trimer/1mg Beads can bind human ACE2 with an EC50 of 1.260 μg/mL.
• Coupled amount of protein: ≈335 pmol (46.8 μg) SARS-CoV-2 (Delta) Spike Trimer/mg beads
• Capacity: >108 pmol (15μg) ACE2/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The SARS-CoV-2 (Delta) Spike Trimer-coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike trimer to streptavidin-conjugated magnetic beads. The spike protein coupled to the beads contains the critical mutations (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N) identified in the SARS-CoV-2 Delta variants (also known as B.1.617.2). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.