Active Spike Trimer (B.1.351) protein-coupled magnetic Beads

• Cat.No.: Spike-4128M

Specification

• Species: COVID-19
• Appearance: Powder mixture
• Particle size: 2 μm
• Source: HEK293
• Protein length: Val 16 - Pro 1213(L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V, R683A, R685A,F817P, A892P, A899P, A942P, K986P, V987P)
• Bio-activity: Immobilized 41μg S protein trimer/1mg beads can bind the Anti-S1 Antibody with an EC50 of 0.5951μg/mL.
• Coupled amount of protein: ≈294 pmol (41 μg) SARS-CoV-2 Spike Trimer (B.1.351)/mg beads
• Capacity: >126 pmol (19 μg) Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The SARS-CoV-2 Spike Trimer (B.1.351) Coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike trimer to streptavidin-conjugated magnetic beads. The spike proteins coupled to the beads contain 10 mutations (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V) identified in the South African variant (known as B.1.351 or 20C/501Y.V2). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.