Active Spike NTD protein-coupled magnetic Beads

• Cat.No.: Spike-4126M

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Protein Length: Val 16 - Phe 318
• Appearance: Powder mixture
• Particle size: 2 μm
• Bio-activity: Immobilized 38 μg NTD protein to 1 mg Beads can bind the Anti-NTD Antibody with an EC50 of 0.7291 μg/mL.
• Coupled amount of protein: ≈684 pmol (26 μg) Spike NTD/mg beads
• Capacity: >65 pmol (10 μg) Anti-NTD antibody/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The SARS-CoV-2 Spike NTD-coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike NTD protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.