Active Recombinant SARS N protein, His-tagged

• Cat.No.: N-0561S
• Product Overview: Recombinant SARS N protein(Met1-Ala422), fused with His tag, was expressed in HEK293.

Specification

• Species: SARS
• Source: HEK293
• Tag: His
• Protein Length: 1-422 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4, with trehalose as protectant.
• Bio-activity: Immobilized SARS Nucleocapsid protein, His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 47.7 kDa. The protein migrates as 30-33 kDa and 55-60 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
• Proteinlength: Met1-Ala422
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.