Active Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y) Protein, His-tagged

• Cat.No.: S-437S
• Product Overview: Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(N501Y)) was expressed in HEK293 cell with a polyhistidine tag at the C-terminus.

Specification

• Species: SARS-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: 319-541
• Predicted N Terminal: Arg 319
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His is 3-20 ng/mL.
  2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein with an affinity constant of 0.827 nM as determined by Octet RED96e system.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
  Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4.
  Please contact us for any concerns or special requirements.
  Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein
• Gene ID: 43740568
• mRNA Refseq: MN908947
• Protein Refseq: YP_009724390