Active Recombinant Rhesus IL10RA Protein (Thr9-Asn245), His-tagged

• Cat.No.: IL10RA-988R
• Product Overview: Recombinant Rhesus IL10RA Protein (XP_001092736.1) (Thr9-Asn245) was produced by HEK293 Cells expression system. This protein was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: Thr9-Asn245
• Description: CD21, also known as IL1RA, is a receptor for interleukin 1. CD21 has been shown to mediate the immunosuppressive signal of interleukin 1, and thus inhibits the synthesis of proinflammatory cytokines. It is structurally related to IFN receptors. It has been reported that CD21 promotes survival of progenitor myeloid cells. Activation of CD21 leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.
• Predicted N Terminal: Phe 29
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit IL-10 dependent proliferation of MC/9-2 mouse mast cells.
  Approximately 0.15-0.6μg/mL of IL-10Rα will inhibit 50% of the biological response induced by 10 ng/mL of recombinant human IL-10.
• Molecular Mass: The recombinant rhesus IL10RA consists of 228 amino acids and predicts a molecular mass of 26.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: IL10RA interleukin 10 receptor subunit alpha [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: IL10RA
• Gene ID: 704383
• mRNA Refseq: XM_001092736
• Protein Refseq: XP_001092736