Active Recombinant Rhesus IL10RA protein, hFc-tagged

• Cat.No.: IL10RA-981R
• Product Overview: Recombinant Rhesus IL10RA protein(XP_001092736.1) (Thr9-Asn245) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: Thr9-Asn245
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  Immobilized Rhesus IL10RA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human IL10, The EC50 of biotinylated human IL10 is 39.0-92.0 ng/ml.
  2. Measured by its ability to inhibit IL-10 dependent proliferation of MC/9 mouse mast cells. Approximately 0.1-0.4 μg/mL of IL-10Rα will inhibit 50% of the biological response induced by 10 ng/mL of recombinant human IL-10.
• Molecular Mass: The recombinant rhesus IL10RA comprises 458 amino acids and has a calculated molecular mass of 51.8 KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.