Active Recombinant Rhesus EFNA5 protein, hFc-tagged

• Cat.No.: EFNA5-130R
• Product Overview: Recombinant Rhesus EFNA5 protein(NP_001252657.1) (Met 1-Asn 203) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: Met 1-Asn 203
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized Rhesus EFNA5-Fc at 10 μg/mL (100 μl/well) can bind biotinylated Rhesus EPHA4. The EC50 of biotinylated Rhesus EPHA4 is 9.3-21.6 ng/mL.
• Molecular Mass: The recombinant rhesus EFNA5 consists of 424 amino acids and has a calculated molecular mass of 48.2 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.