Active Recombinant Rhesus ACE2 protein, hFc-tagged

• Cat.No.: ACE2-207R
• Product Overview: Recombinant Rhesus ACE2 protein(ACI04564.1)(Met1-Val739), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Val739
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK (Dnp)-OH. The specific activity is >250 pmol/min/µg.2. Immobilized SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) at 2μg/mL (100μL/well) can bind Rhesus ACE2-Fc,the EC50 of ACE2 is 30-80 ng/mL.
• Molecular Mass: The recombinant Rhesus ACE2 is comprises 963 amino acids and has a calculated molecular mass of 110.7kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.