Active Recombinant Rat Vegfa protein(Met1-Arg190)

Source :	Insect Cells
Species :	Rat
Tag :	Non
Protein length :	Met1-Arg190
Form :	Lyophilized from sterile 100mM Glycine, 10mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity :	Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-10 ng/ml.
Molecular Mass :	The secreted recombinant rat VEGF164 consists of 164 amino acids and has a predicted molecular mass of 19.2 kDa. The apparent molecular mass of the rat VEGF164 is approximately 25 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin :	< 1.0 EU per μg of the protein as determined by the LAL method
Purity :	> 86 % as determined by SDS-PAGE
Storage :	Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	Vegfa vascular endothelial growth factor A [ Rattus norvegicus ]
Official Symbol	Vegfa
Synonyms	VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164;
Gene ID	83785
mRNA Refseq	NM_001110333
Protein Refseq	NP_001103803