Active Recombinant Rat Tnfsf9 protein, hFc-tagged

• Cat.No.: Tnfsf9-7445R
• Product Overview: Recombinant Rat Tnfsf9 protein(Q80WE6)(Pro106-Gly308), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: 106-308 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized canine TNFRSF9-His at 10 μg/ml (100 μl/well) can bind rat Fc-TNFSF9, The EC50 of rat Fc-TNFSF9 is 0.26-0.62 μg/ml.
• Molecular Mass: The recombinant rat TNFSF9 comprises 463 amino acids and predicts a molecular mass of 50.9 kDa. The apparent molecular mass of the recombinant protein is approximately 61 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Proteinlength: Pro106-Gly308
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfsf9 tumor necrosis factor (ligand) superfamily, member 9 [ Rattus norvegicus ]
• Official Symbol: Tnfsf9
• Synonyms: TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; costimulatory molecule
• Gene ID: 353218
• mRNA Refseq: NM_181384
• Protein Refseq: NP_852049