Active Recombinant Rat TNFSF11 protein, His-Avi-tagged, Biotinylated

• Cat.No.: TNFSF11-0754R
• Product Overview: Biotinylated Recombinant Rat TNFSF11 protein(Leu69-Asp318), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Avi&His
• Protein Length: 69-318 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin at 1 μg/mL (100 μL/well) can bind Biotinylated Rat TNFSF11, His,Avitag with a linear range of 0.5-16 ng/mL. Immobilized Biotinylated Rat TNFSF11, His,Avitag at 2 μg/mL (100 μL/well) on streptavidin precoated (1 μg/well) plate can bind Human Osteoprotegerin, Fc Tag with a linear range of 0.1-16 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 31.8 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu69-Asp318
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfsf11 tumor necrosis factor (ligand) superfamily, member 11 [ Rattus norvegicus ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; ODF; OPGL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RANKL
• Gene ID: 117516
• mRNA Refseq: NM_057149
• Protein Refseq: NP_476490