Active Recombinant Rat SIRPA protein, Fc-tagged

• Cat.No.: SIRPA-0697R
• Product Overview: Recombinant Rat SIRPA protein(Lys32-Asn373), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: 32-373 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat SIRP alpha, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 1-20 ng/mL. Immobilized Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Rat SIRP alpha, Fc Tag with a linear range of 2-39 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 64.0 kDa. The protein migrates as 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Lys32-Asn373
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sirpa signal-regulatory protein alpha [ Rattus norvegicus ]
• Official Symbol: SIRPA
• Synonyms: SIRPA; signal-regulatory protein alpha; tyrosine-protein phosphatase non-receptor type substrate 1; sirp-alpha-1; SHP substrate 1; inhibitory receptor SHPS-1; macrophage fusion receptor; signal-regulatory protein alpha-1; CD172 antigen-like family member A; macrophage membrane protein MFP150; brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like protein with tyrosine - based activation motifs; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); Bit; Ptpns1; SHPS-1
• Gene ID: 25528
• mRNA Refseq: NM_013016
• Protein Refseq: NP_037148