Active Recombinant Rat Rps6kb1(T412E), His-tagged

• Cat.No.: Rps6kb1-143R
• Product Overview: Recombinant Rat Rps6kb1 (amino acid residues 1-421), with N-terminal 6His, was expressed in insect cells. p70 S6 kinase has a T412E mutation to mimic the activation of the enzyme through phosphorylation of Thr412 by PDK2.

Specification

• Description: Protein ubiquitylation and protein phosphorylation are the two major mechanisms that regulate the functions of proteins in eukaryotic cells.
• Source: Insect cells
• Species: Rat
• Tag: His
• Form: 50 mM Tris/HCl pH7.5, 0.1mM EGTA, 150 mM NaCl, 0.1% ?-Mercaptoethanol, 270 mM sucrose, 0.03% Brij-35, 1 mM Benzamidine, 0.2 mM PMSF.
• Bio-activity: 154.2 Units/mg (169.6 Units/ml)Substrate: CROSStide (GRPRTSSFAEG)
• Molecular Mass: ~48.6 kDa
• Purity: >95% by InstantBlue™ SDS-PAGE
• Unit Definition: 1 Unit = 1 nmole of phosphate incorporated into the substrate in 1 minute.
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 1.1 mg/ml
• Protein length: 1-421 a.a.

Gene Information

• Gene Name: Rps6kb1 ribosomal protein S6 kinase, polypeptide 1 [ Rattus norvegicus ]
• Official Symbol: Rps6kb1
• Synonyms: RPS6KB1; ribosomal protein S6 kinase, polypeptide 1; ribosomal protein S6 kinase beta-1; S6K; S6K1; P70S6K; P70S6K1; p70 S6KA; S6 kinase; p70-S6K 1; S6K-beta-1; p70 S6K-alpha; p70(S6K)-alpha; p70 S6 kinase alpha; p70 ribosomal S6 kinase alpha; ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Gene ID: 83840
• mRNA Refseq: NM_031985
• Protein Refseq: NP_114191
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem
• Function: ATP binding; ATP binding; nucleotide binding; peptide binding; protein binding; protein kinase activity; protein kinase activity; protein kinase activity; ribosomal protein S6 kinase activity; transferase activity, transferring phosphorus-containing groups