Active Recombinant Rat PDCD1 protein(Met1-Gln167), hFc-tagged

• Cat.No.: PDCD1-1033R
• Product Overview: Recombinant Rat PDCD1 (NP_001100397.1)(Met1-Gln167) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Protein length: Met1-Gln167
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized PD-1 Protein, Rat, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind PD-L2 Protein, Mouse, Recombinant (His Tag), the EC50 is 120-780 ng/mL.
• Molecular Mass: The recombinant Rat PDCD1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 384 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately118.8 and 58.4 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pdcd1 programmed cell death 1 [ Rattus norvegicus ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1
• Gene ID: 301626
• mRNA Refseq: NM_001106927
• Protein Refseq: NP_001100397