Active Recombinant Rat NTRK2 Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: NTRK2-1095RAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant rat NTRK2 (Q63604-Isoform T1) (Met1-His429) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• ProteinLength: 409
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rat NTRK2-His at 10 μg/mL (100 μL/well) can bind biotinylated mouse BDNF, The EC50 of biotinylated mouse BDNF is 12.2-28.6 ng/mL.
• Molecular Mass: The recombinant rat NTRK2 comprises 409 amino acids and predicts a molecular mass of 47.1 kDa. The apparent molecular mass of the recombinant protein is approximately 67 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• N-terminal Sequence Analysis: Cys 32
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Ntrk2 neurotrophic tyrosine kinase, receptor, type 2 [ Rattus norvegicus ]
• Official Symbol: NTRK2
• Synonyms: NTRK2; neurotrophic tyrosine kinase, receptor, type 2; BDNF/NT-3 growth factors receptor; trk-B; GP145-TrkB/GP95-TrkB; trkB tyrosine kinase; Neural receptor protein-tyrosine kinase (trkB); Tkrb; trkB; TRKB1; RATTRKB1
• Gene ID: 25054
• mRNA Refseq: NM_001163168
• Protein Refseq: NP_001156640