Active Recombinant Rat IL6ST protein, His&mFc-tagged

• Cat.No.: IL6ST-1964R
• Product Overview: Recombinant Rat IL6ST protein(NP_001008725.2)(Met4-Glu618), fused with His&mFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His&mFc
• Protein Length: Met4-Glu618
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED50 for this effect is typically 0.015-0.075 μg/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6.
• Molecular Mass: The recombinant rat IL6ST/Fc is a disulfide-linked homodimer. The reduced monomer comprises 839 amino acids and predicts a molecular mass of 94.3 kDa. The apparent molecular mass of the rat IL6ST/Fc monomer is approximately 130 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il6st interleukin 6 signal transducer [ Rattus norvegicus ]
• Official Symbol: IL6ST
• Synonyms: IL6ST; interleukin 6 signal transducer; interleukin-6 receptor subunit beta; IL-6R subunit beta; membrane glycoprotein 130; IL-6 receptor subunit beta; interleukin-6 signal transducer; oncostatin-M receptor subunit alpha; Gp130; Ac1055; Il-6rb
• Gene ID: 25205
• mRNA Refseq: NM_001008725
• Protein Refseq: NP_001008725