Source : |
E. coli |
Species : |
Human |
Tag : |
Non |
Form : |
Lyophilized from sterile 20mM Tris, 20mM NaCl, pH 6.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
1. Immobilized human IL10 at 10 μg/mL (100 μl/well) can bind Cynomolgus IL10RA-Fc, The EC50 of Cynomolgus IL10RA-Fc is 0.24-0.56 μg/mL.2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells.The ED50 for this effect is typically 0.5-2.5 ng/mL. |
Molecular Mass : |
The recombinant human GADD45G consists of 160 amino acids and predicts a molecular mass of 18.6 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions. |
Protein length : |
Ser 19-Asn 178 |
Purity : |
> 95 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |