Active Recombinant Rat Flt1 protein, His-tagged

• Cat.No.: Flt1-8712R
• Product Overview: Recombinant Rat Flt1 protein(P53767)(Met1-Glu758), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Glu758
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 15-70ng/mL in the presence of 10 ng/mL rmVEGF164.
• Molecular Mass: The recombinant rat FLT1 comprises 747 amino acids and predicts a molecular mass of 84 kDa. The apparent molecular mass of the recombinant protein is approximately 108-114 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Flt1 FMS-related tyrosine kinase 1 [ Rattus norvegicus ]
• Official Symbol: Flt1
• Synonyms: FLT1; FMS-related tyrosine kinase 1; vascular endothelial growth factor receptor 1; FLT-1; FMS-like tyrosine kinase 1; tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor-1; Fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); VEGFR-1
• Gene ID: 54251
• mRNA Refseq: NM_019306
• Protein Refseq: NP_062179