Active Recombinant Rat Fcgr3a protein, His-tagged

• Cat.No.: Fcgr3a-4082R
• Product Overview: Recombinant Rat Fcgr3a protein(ABO70343.1)(Met1-Pro200), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Pro200
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rat FCGR3A-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 59.0-139.0 ng/ml.
• Molecular Mass: The recombinant rat FCGR3A comprises 190 amino acids and predicts a molecular mass of 21.7 kDa. The apparent molecular mass of the rat FCGR3A is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgr3a Fc fragment of IgG, low affinity IIIa, receptor [ Rattus norvegicus ]
• Official Symbol: Fcgr3a
• Synonyms: FCGR3A; Fc fragment of IgG, low affinity IIIa, receptor; transmembrane receptor FcgammaRIII-X; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fcgr4
• Gene ID: 304966
• mRNA Refseq: NM_207603
• Protein Refseq: NP_997486