Active Recombinant Rat Erbb4 protein(Met1-Pro651), hFc-tagged

• Cat.No.: Erbb4-8720R
• Product Overview: Recombinant Rat ERBB4 (AAQ77349.1)(Met1-Pro651) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Pro651
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Rat ERBB4-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1, The EC50 of biotinylated human NRG1 is 0.69-1.61 μg/ml.
• Molecular Mass: The recombinant Rat ERBB4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 867 amino acids and has a predicted molecular mass of 96.9 kDa. The apparent molecular mass of the protein is approximately 119 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Erbb4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) [ Rattus norvegicus ]
• Official Symbol: Erbb4
• Synonyms: ERBB4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); receptor tyrosine-protein kinase erbB-4; c-erbB-4; receptor tyrosine kinase; proto-oncogene-like protein c-ErbB-4
• Gene ID: 59323
• mRNA Refseq: NM_021687
• Protein Refseq: NP_067719