Active Recombinant Rat Epha3 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Epha3-551RAF555
• Product Overview: A DNA sequence encoding the rat EPHA3 (EDL75897.1) (Met1-His541) (Alexa Fluor 555 conjugated) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-His541， 762
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized ratEFNA5-His at 10 μg/mL (100 μL/well) can bind rat EPHA3-Fc, The EC50 of rat EPHA3-Fc is 12.1-28.4 ng/mL.
• Molecular Mass: The recombinant rat EPHA3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 762 amino acids and has a predicted molecular mass of 85.8 kDa. The apparent molecular mass of the protein is approximately 103 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: Epha3 Eph receptor A3 [ Rattus norvegicus ]
• Official Symbol: Epha3
• Synonyms: EPHA3; Eph receptor A3; ephrin type-A receptor 3; EK4; rEK4; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor REK4
• Gene ID: 29210
• mRNA Refseq: NM_031564
• Protein Refseq: NP_113752