Active Recombinant Rat Efnb3 protein, His-tagged

• Cat.No.: Efnb3-8717R
• Product Overview: Recombinant Rat Efnb3 protein(G3V7D4)(Met1-Ser224), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser224
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized rat EFNB3-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His, The EC50 of biotinylated mouse EphB3-His is 17.4-40.6 ng/ml.
• Molecular Mass: The recombinant rat EFNB3 comprises 208 amino acids and predicts a molecular mass of 23.1 kDa. The apparent molecular mass of the recombinant protein is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb3 ephrin B3 [ Rattus norvegicus ]
• Official Symbol: Efnb3
• Synonyms: EFNB3; ephrin B3; ephrin ligand B3; ELK-L3; RGD1565678
• Gene ID: 360546
• mRNA Refseq: NM_001100980
• Protein Refseq: NP_001094450