Active Recombinant Rat Efna5 protein, His-tagged

• Cat.No.: Efna5-1620R
• Product Overview: Recombinant Rat Efna5(Met 1-Glu 202) fused with His tag at C-terminal was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Glu 202
• Predicted N Terminal: Gln 21
• Form: Lyophilized from sterile PBS, pH 7.51. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized rat EFNA5-His at 10 μg/ml (100 μl/well) can bind rat EPHA3-Fc, The EC50 of rat EPHA3-Fc is 12.1-28.3 ng/ml.
• Molecular Mass: The recombinant rat EFNA5 comprises 193 amino acids and predicts a molecular mass of 22.5 kDa. The apparent molecular mass of the ratEFNA5 is approximately 27 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Efna5 ephrin A5 [ Rattus norvegicus ]
• Official Symbol: Efna5
• Synonyms: EFNA5; ephrin A5; ephrin-A5; AL-1; LERK-7; eph-related receptor tyrosine kinase ligand 7; Lerk7
• Gene ID: 116683
• mRNA Refseq: NM_053903
• Protein Refseq: NP_446355
• UniProt ID: P97605
• Chromosome Location: 9q37
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: chemorepellent activity; chemorepellent activity; ephrin receptor binding; ephrin receptor binding; ephrin receptor binding