Active Recombinant Rat Cd70 protein, His-tagged

• Cat.No.: Cd70-8731R
• Product Overview: Recombinant Rat Cd70 protein(NP_001100348)(Gln46-Pro195), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA.2.Immobilized rat His-CD70 at 10μg/mL (100μL/well) can bind mouse CD27-Fch, the EC50 of mouse CD27-Fch is 10-50ng/mL.
• Molecular Mass: The recombinant rat CD70 comprises 170 amino acids and has a predicted molecular mass of 18.9 kDa. The apparent molecular mass of the protein is approximately 28 kDa in SDS-PAGE under reducing conditions.
• Protein length: Gln46-Pro195
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd70 Cd70 molecule [ Rattus norvegicus ]
• Official Symbol: Cd70
• Synonyms: CD70; Cd70 molecule; CD70 antigen
• Gene ID: 301132
• mRNA Refseq: NM_001106878
• Protein Refseq: NP_001100348