Active Recombinant Rat Cd44 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd44-1017RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Rat Cd44 (Accession # O70509), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Rat CD44 Fc Chimera is present at 1 μg/mL, the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 10-50 ng/mL.
• Molecular Mass: Recombinant Rat Cd44, Fc Chimera has a calculated MW of 54.2 kDa (monomer). In SDS-PAGE migrates as 80-100 kDa, reducing conditions.
• N-terminal Sequence Analysis: Result not obtained, Gln 22 is predicted
• Purity: > 95 % by SDS-PAGE under reducing conditions and visualized by silver stain.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Concentration: 500 μg/mL
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: Cd44 Cd44 molecule [ Rattus norvegicus ]
• Official Symbol: Cd44
• Synonyms: CD44; Cd44 molecule; CD44 antigen; PGP-1; PGP-I; HUTCH-I; ECMR-III; hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; phagocytic glycoprotein I; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Cell surface glycoprotein CD44 (hyaluronate binding protein); CD44A; METAA; RHAMM; MGC124941
• Gene ID: 25406
• mRNA Refseq: NM_012924
• Protein Refseq: NP_037056