Active Recombinant Rat Adam17 protein(Met1-Asp563), His-tagged

• Cat.No.: Adam17-8788R
• Product Overview: Recombinant Rat ADAM17 (Q9Z1K9) (Met1-Asp563) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Asp563
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003). The specific activity is >250 pmol/min/μg.
• Molecular Mass: The recombinant rat ADAM17 comprises 557 amino acids and predicts a molecular mass of 63 kDa. The apparent molecular mass of the recombinant protein is approximately 57 and 20 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Adam17 ADAM metallopeptidase domain 17 [ Rattus norvegicus ]
• Official Symbol: Adam17
• Synonyms: ADAM17; ADAM metallopeptidase domain 17; disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; a disintegrin and metalloprotease domain 17; a disintegrin and metallopeptidase domain 17; a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); TACE
• Gene ID: 57027
• mRNA Refseq: NM_020306
• Protein Refseq: NP_064702