Active Recombinant Porcine endoglin Protein, Fc tagged

• Cat.No.: ENG-13P
• Product Overview: Recombinant Porcine Endoglin protein (Val29-Gly581) with Human IgG1 Fc tag (Pro100-Lys330) at C-terminus was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Porcine
• Source: CHO
• Tag: Fc
• Description: Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature. Regulates the migration of vascular endothelial cells.
• Tag: Fc
• Form: Disulfide-linked homodimer
• Molecular Mass: 86.8 kDa (monomer)
• Bio-activity: Measured by its ability to inhibit BMP-10-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 0.3-1.2 μg/mL.
• N-terminal Sequence Analysis: Val29
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reference: 1. Yamashita, H. et al. (1994) J. Biol. Chem. 269:1995.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.

Gene Information

• Gene Name: ENG endoglin [ Sus scrofa (pig) ]
• Official Symbol: ENG
• Synonyms: ENG; endoglin; CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1
• Gene ID: 397096
• mRNA Refseq: NM_214031
• Protein Refseq: NP_999196
• UniProt ID: P37176