Active Recombinant Pig IL10 protein

• Cat.No.: IL10-569P
• Product Overview: Recombinant Pig IL10 protein(Q29055)(Ser19-Asn175) was expressed in E. coli.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• Protein Length: 19-175 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized sus scrofa (pig) IL10 at 2 μg/ml (100 μl/well) can bind Rhesus IL10RA-hFc, the EC50 of Rhesus IL10RA-hFc is 25-100 ng/mL.2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 1-5 ng/mL.
• Molecular Mass: The recombinant porcine IL10 consists of 158 amino acids and has a predicted molecular mass of 18.2 kDa. The apparent molecular mass of it is approximately 18 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Ser19-Asn175
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL10 interleukin 10 [ Sus scrofa ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; IL-10
• Gene ID: 397106
• mRNA Refseq: NM_214041
• Protein Refseq: NP_999206