Active Recombinant Pig CYP2E1 Protein

Cat.No. : CYP2E1-134P
Product Overview : Recombinant pig CYP2E1 protein and CYP-reductase without tag were coexpressed in E. coli.
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Species : Pig
Source : E.coli
Description : A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
Bio-activity : Cytochrome c Reductase Activity: 367 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Chlorzoxazone 6-hydroxylase
Linear with time up to: 15 min(Determined at 100 µM substrate, using a P450 concentration of 100 pmol/ml)
Linear with P450 up to: 160 pmol/ml(Determined at 100 µM substrate, using an incubation time of 5 min)
Vmax: 3.2 pmol/min/pmol CYP
Km: 132 µM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 9.9 nmol/ml
Cytochrome b5 Concentration: 49.4 nmol/ml
Protein Concentration: 17.7 mg/ml
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Sus scrofa (pig) ]
Official Symbol CYP2E1
Synonyms CYP2E1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P-450-J; cytochrome P450-J
Gene ID 403216
mRNA Refseq NM_214421
Protein Refseq NP_999586
UniProt ID P79383

Not For Human Consumption!

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