Active Recombinant Pig CYP2E1 Protein
Cat.No. : | CYP2E1-134P |
Product Overview : | Recombinant pig CYP2E1 protein and CYP-reductase without tag were coexpressed in E. coli. |
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Species : | Pig |
Source : | E.coli |
Description : | A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics. |
Bio-activity : | Cytochrome c Reductase Activity: 367 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: Chlorzoxazone 6-hydroxylase Linear with time up to: 15 min(Determined at 100 µM substrate, using a P450 concentration of 100 pmol/ml) Linear with P450 up to: 160 pmol/ml(Determined at 100 µM substrate, using an incubation time of 5 min) Vmax: 3.2 pmol/min/pmol CYP Km: 132 µM |
Usage : | For laboratory (research) purposes only |
Storage : | Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
Concentration : | P450 Concentration: 9.9 nmol/ml Cytochrome b5 Concentration: 49.4 nmol/ml Protein Concentration: 17.7 mg/ml |
Storage Buffer : | 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
Gene Name | CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Sus scrofa (pig) ] |
Official Symbol | CYP2E1 |
Synonyms | CYP2E1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P-450-J; cytochrome P450-J |
Gene ID | 403216 |
mRNA Refseq | NM_214421 |
Protein Refseq | NP_999586 |
UniProt ID | P79383 |
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◆ Cell & Tissue Lysates | ||
CYP2E1-7111HCL | Recombinant Human CYP2E1 293 Cell Lysate | +Inquiry |
Not For Human Consumption!
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