Active Recombinant Mouse Wnt-1/sFRP-1 Complex Protein

• Cat.No.: Wnt1-001M
• Product Overview: Recombinant mouse Wnt-1 (Ala28-Leu370) and sFRP-1 (Ser32-Lys314) complex protein without tag was expressed in Chinese hamster ovary cell.

Specification

• Species: Mouse
• Source: CHO
• Tag: Non
• Description: Biased expression in CNS E11.5 (RPKM 2.1), testis adult (RPKM 1.8) and 5 other tissues.
• Bio-activity: Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.03-0.18 μg/mL
• Molecular Mass: 38 kDa (Wnt-1) & 33 kDa (sFRP-1)
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Trehalose and with BSA as a carrier protein.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Wnt1 wingless-type MMTV integration site family, member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Wnt1
• Synonyms: Wnt1; wingless-type MMTV integration site family, member 1; sw; Int-1; Wnt-1; swaying; proto-oncogene Wnt-1; proto-oncogene Int-1; proto-oncogene protein Wnt-1; wingless-related MMTV integration site 1
• Gene ID: 22408
• mRNA Refseq: NM_021279
• Protein Refseq: NP_067254
• UniProt ID: P04426