Active Recombinant Mouse Vegfa Protein (Ala205-Arg368), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Vegfa-92M
• Product Overview: Recombinant Mouse Vegfa Protein (Ala205-Arg368) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Vascular Endothelial Growth Factors 165 (VEGF165) is a potent growth and angiogenic cytokine which belongs to the VEGF family, includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains. VEGF165 plays an important role in embryonic vasculogenesis, angiogenesis and neurogenesis.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is < 3 ng/mL.
• Molecular Mass: The protein has a calculated MW of 20.22 kDa.
  The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Ala205-Arg368
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Mus musculus (house mouse) ]
• Official Symbol: Vegfa
• Synonyms: Vegfa; vascular endothelial growth factor A; Vpf; Vegf; L-VEGF; vascular endothelial growth factor A, long form; vascular permeability factor
• Gene ID: 22339
• mRNA Refseq: NM_009505
• Protein Refseq: NP_033531
• UniProt ID: F8WH10