Active Recombinant Mouse Tumor Necrosis Factor

• Cat.No.: Tnf-310M
• Product Overview: Recombinant murine TNF-alpha produced in S.Lividians is a single,non-glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32 Dalton.

Specification

• Species: Mouse
• Source: Streptomyces Lividans
• Tag: Non
• Description: Tumor necrosis factor (TNF, cachexin or cachectin formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction.
• Form: Each mg contains 50mM Tris-HCL pH-8 and 20mM NaCL.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108IU/mg.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Amino acid sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
• Pathways: Adipocytokine signaling pathway; Adipogenesis; African trypanosomiasis; Allograft rejection; Alzheimer"s disease; Amoebiasis; Antigen processing and presentation; Apoptosis; Asthma; Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response (BioCarta); Death Receptor Signalling; Diabetes pathways; Dilated cardiomyopathy; Extrinsic Pathway for Apoptosis; Fc epsilon RI signaling pathway; Tuberculosis; Type I diabetes mellitus; Type II diabetes mellitus

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Mus musculus ]
• Official Symbol: Tnf
• Synonyms: Tnf; tumor necrosis factor; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; MGC151434; TNF-alpha; TNF-a; TNF alpha; cachectin; tumor necrosis factor alpha;tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; APC1 protein; cachectin; TNF superfamily, member 2; TNFA; TNF, macrophage-derived; TNF, monocyte-derived; tumor necrosis factor-alpha; tumor necrosis factor (TNF superfamily, member 2); Cachectin
• Gene ID: 21926
• mRNA Refseq: NM_013693
• Protein Refseq: NP_038721
• UniProt ID: P06804
• Chromosome Location: 17 B1; 17 19.06 cM
• Function: cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding